Ontology highlight
ABSTRACT:
SUBMITTER: Guryanova OA
PROVIDER: S-EPMC2748426 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Guryanova O A OA Makhanov M M Chenchik A A AA Chumakov P M PM Frolova E I EI
Molecular biology 20060501 3
To obtain a whole genome library that suppresses the total diversity of human mRNAs, lentiviral vector constructs and a short hairpin RNA (shRNA) expression cassette were optimized. The optimization of the vector increased the virus titer in preparations by 15-20 times. A simple shRNA structure with a 21-bp stem proved to be the most effective. Lentivector-based shRNA expression constructs were obtained by using puro(R), copGFP, or H-2K(k) as a selectable marker. The efficiency of the optimized ...[more]