Dataset Information

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

ABSTRACT:

Background

RNA interference (RNAi) is a powerful approach to study a gene function. Transgenic RNAi is an adaptation of this approach where suppression of a specific gene is achieved by expression of an RNA hairpin from a transgene. In somatic cells, where a long double-stranded RNA (dsRNA) longer than 30 base-pairs can induce a sequence-independent interferon response, short hairpin RNA (shRNA) expression is used to induce RNAi. In contrast, transgenic RNAi in the oocyte routinely employs a long RNA hairpin. Transgenic RNAi based on long hairpin RNA, although robust and successful, is restricted to a few cell types, where long double-stranded RNA does not induce sequence-independent responses. Transgenic RNAi in mouse oocytes based on a shRNA offers several potential advantages, including simple cloning of the transgenic vector and an ability to use the same targeting construct in any cell type.

Results

Here we report our experience with shRNA-based transgenic RNAi in mouse oocytes. Despite optimal starting conditions for this experiment, we experienced several setbacks, which outweigh potential benefits of the shRNA system. First, obtaining an efficient shRNA is potentially a time-consuming and expensive task. Second, we observed that our transgene, which was based on a common commercial vector, was readily silenced in transgenic animals.

Conclusions

We conclude that, the long RNA hairpin-based RNAi is more reliable and cost-effective and we recommend it as a method-of-choice when a gene is studied selectively in the oocyte.

SUBMITTER: Sarnova L

PROVIDER: S-EPMC2964603 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Publications

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

Sarnova Lenka L Malik Radek R Sedlacek Radislav R Svoboda Petr P

Journal of negative results in biomedicine 20101012

<h4>Background</h4>RNA interference (RNAi) is a powerful approach to study a gene function. Transgenic RNAi is an adaptation of this approach where suppression of a specific gene is achieved by expression of an RNA hairpin from a transgene. In somatic cells, where a long double-stranded RNA (dsRNA) longer than 30 base-pairs can induce a sequence-independent interferon response, short hairpin RNA (shRNA) expression is used to induce RNAi. In contrast, transgenic RNAi in the oocyte routinely emplo ...[more]

PMID: 20939886

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Dataset Information

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

Background

Results

Conclusions

Publications

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

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