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MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats.

ABSTRACT: BACKGROUND: The brain is a major site of microRNA (miRNA) gene expression, but the spatial expression patterns of miRNAs within the brain have not yet been fully covered. METHODOLOGY/PRINCIPAL FINDINGS: We have characterized the regional expression profiles of miRNAs in five distinct regions of the adult rat brain: amygdala, cerebellum, hippocampus, hypothalamus and substantia nigra. Microarray profiling uncovered 48 miRNAs displaying more than three-fold enrichment between two or more brain regions. Notably, we found reciprocal expression profiles for a subset of the miRNAs predominantly found (> ten times) in either the cerebellum (miR-206 and miR-497) or the forebrain regions (miR-132, miR-212, miR-221 and miR-222). CONCLUSIONS/SIGNIFICANCE: The results indicate that some miRNAs could be important for area-specific functions in the brain. Our data, combined with previous studies in mice, provides additional guidance for future investigations of miRNA functions in the brain.

SUBMITTER: Olsen L

PROVIDER: S-EPMC2752988 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats.

Olsen Line L Klausen Mikkel M Helboe Lone L Nielsen Finn Cilius FC Werge Thomas T

PloS one 20091006 10

<h4>Background</h4>The brain is a major site of microRNA (miRNA) gene expression, but the spatial expression patterns of miRNAs within the brain have not yet been fully covered.<h4>Methodology/principal findings</h4>We have characterized the regional expression profiles of miRNAs in five distinct regions of the adult rat brain: amygdala, cerebellum, hippocampus, hypothalamus and substantia nigra. Microarray profiling uncovered 48 miRNAs displaying more than three-fold enrichment between two or m ...[more]

PMID: 19806225

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