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Genetic basis for alkaline activation of germination in Bacillus thuringiensis subsp. israelensis.


ABSTRACT: Differences in activation between spores from strains of Bacillus thuringiensis subsp. israelensis with and without the toxin-encoding plasmid pBtoxis are demonstrated. Following alkaline activation, the strain bearing pBtoxis shows a significantly greater germination rate. Expression of just three genes constituting a previously identified, putative ger operon from this plasmid is sufficient to produce the same phenotype and characterizes this operon as a genetic determinant of alkaline activation.

SUBMITTER: Abdoarrahem MM 

PROVIDER: S-EPMC2753073 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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Genetic basis for alkaline activation of germination in Bacillus thuringiensis subsp. israelensis.

Abdoarrahem M M MM   Gammon K K   Dancer B N BN   Berry C C  

Applied and environmental microbiology 20090731 19


Differences in activation between spores from strains of Bacillus thuringiensis subsp. israelensis with and without the toxin-encoding plasmid pBtoxis are demonstrated. Following alkaline activation, the strain bearing pBtoxis shows a significantly greater germination rate. Expression of just three genes constituting a previously identified, putative ger operon from this plasmid is sufficient to produce the same phenotype and characterizes this operon as a genetic determinant of alkaline activat  ...[more]

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