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Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes.

ABSTRACT: Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.

SUBMITTER: Kudva IT

PROVIDER: S-EPMC2754196 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

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Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes.

Kudva Indira T IT Krastins Bryan B Sheng Haiqing H Griffin Robert W RW Sarracino David A DA Tarr Phillip I PI Hovde Carolyn J CJ Calderwood Stephen B SB John Manohar M

Molecular & cellular proteomics : MCP 20060531 8

Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal f ...[more]

PMID: 16737953

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