Ontology highlight
ABSTRACT:
SUBMITTER: Xu H
PROVIDER: S-EPMC2754248 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Xu Hong H DeLuca Steven Z SZ O'Farrell Patrick H PH
Science (New York, N.Y.) 20080701 5888
High copy number and random segregation confound genetic analysis of the mitochondrial genome. We developed an efficient selection for heritable mitochondrial genome (mtDNA) mutations in Drosophila, thereby enhancing a metazoan model for study of mitochondrial genetics and mutations causing human mitochondrial disease. Targeting a restriction enzyme to mitochondria in the germline compromised fertility, but escaper progeny carried homoplasmic mtDNA mutations lacking the cleavage site. Among muta ...[more]