Ontology highlight
ABSTRACT:
SUBMITTER: Spitale RC
PROVIDER: S-EPMC2754253 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Spitale Robert C RC Volpini Rosaria R Mungillo Michael V MV Krucinska Jolanta J Cristalli Gloria G Wedekind Joseph E JE
Biochemistry 20090801 33
The hairpin ribozyme cleaves a phosphodiester bond within a cognate substrate. Structural and biochemical data indicate the conserved A9 and A10 bases reside close to the scissile bond but make distinct contributions to catalysis. To investigate these residues, we replaced the imino moiety of each base with N1-deazaadenosine. This single-atom change resulted in an 8-fold loss in k(obs) for A9 and displacement of the base from the active site; no effects were observed for A10. We propose that the ...[more]