Energetics of echinomycin binding to DNA.
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ABSTRACT:
SUBMITTER: Leng F
PROVIDER: S-EPMC275468 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
Publications
Energetics of echinomycin binding to DNA.
Leng Fenfei F Chaires Jonathan B JB Waring Michael J MJ
Nucleic acids research 20031101 21
Differential scanning calorimetry and UV thermal denaturation have been used to determine a complete thermodynamic profile for the bis-intercalative interaction of the peptide antibiotic echinomycin with DNA. The new calorimetric data are consistent with all previously published binding data, and afford the most rigorous and direct determination of the binding enthalpy possible. For the association of echinomycin with DNA, we found DeltaG degrees = -7.6 kcal mol(-1), DeltaH = +3.8 kcal mol(-1) a ...[more]
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