Ontology highlight
ABSTRACT:
SUBMITTER: Perales C
PROVIDER: S-EPMC275559 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
Nucleic acids research 20031101 22
Bacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from Thermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA specifically over a temperature range of 4-80 degrees C, and the wild-type protein could withstand incubation at 94 degrees C for 2 min. Addition of TthSSB to PCR halved the elong ...[more]