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Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.

ABSTRACT: Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.

SUBMITTER: Hohmann-Marriott MF

PROVIDER: S-EPMC2755602 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.

Hohmann-Marriott Martin F MF Sousa Alioscka A AA Azari Afrouz A AA Glushakova Svetlana S Zhang Guofeng G Zimmerberg Joshua J Leapman Richard D RD

Nature methods 20090830 10

Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human er ...[more]

PMID: 19718033

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