Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.

ABSTRACT: Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.

SUBMITTER: Hohmann-Marriott MF 

PROVIDER: S-EPMC2755602 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature