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Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.


ABSTRACT: Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.

SUBMITTER: Hohmann-Marriott MF 

PROVIDER: S-EPMC2755602 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.

Hohmann-Marriott Martin F MF   Sousa Alioscka A AA   Azari Afrouz A AA   Glushakova Svetlana S   Zhang Guofeng G   Zimmerberg Joshua J   Leapman Richard D RD  

Nature methods 20090830 10


Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human er  ...[more]

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