Ontology highlight
ABSTRACT:
SUBMITTER: Dengl S
PROVIDER: S-EPMC2755851 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20090617 32
Termination of RNA polymerase (pol) II transcription in vivo requires the 5'-RNA exonuclease Rat1. It was proposed that Rat1 degrades RNA from the 5'-end that is created by transcript cleavage, catches up with elongating pol II, and acts like a Torpedo that removes pol II from DNA. Here we test the Torpedo model in an in vitro system based on bead-coupled pol II elongation complexes (ECs). Recombinant Rat1 complexes with Rai1, and with Rai1 and Rtt103, degrade RNA extending from the EC until the ...[more]