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Touch induces ATP release in Arabidopsis roots that is modulated by the heterotrimeric G-protein complex.

ABSTRACT: Amongst the many stimuli orienting the growth of plant roots, of critical importance are the touch signals generated as roots explore the mechanically complex soil environment. However, the molecular mechanisms behind these sensory events remain poorly defined. We report an impaired obstacle-avoiding response of roots in Arabidopsis lacking a heterotrimeric G-protein. Obstacle avoidance may utilize a touch-induced release of ATP to the extracellular space. While sequential touch stimulation revealed a strong refractory period for ATP release in response to mechano-stimulation in wild-type plants, the refractory period in mutants was attenuated, resulting in extracellular ATP accumulation. We propose that ATP acts as an extracellular signal released by mechano-stimulation and that the G-protein complex is needed for fine-tuning this response.

SUBMITTER: Weerasinghe RR

PROVIDER: S-EPMC2756610 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Touch induces ATP release in Arabidopsis roots that is modulated by the heterotrimeric G-protein complex.

Weerasinghe Ravisha R RR Swanson Sarah J SJ Okada Seiko F SF Garrett Michele B MB Kim Sung-Yong SY Stacey Gary G Boucher Richard C RC Gilroy Simon S Jones Alan M AM

FEBS letters 20090715 15

Amongst the many stimuli orienting the growth of plant roots, of critical importance are the touch signals generated as roots explore the mechanically complex soil environment. However, the molecular mechanisms behind these sensory events remain poorly defined. We report an impaired obstacle-avoiding response of roots in Arabidopsis lacking a heterotrimeric G-protein. Obstacle avoidance may utilize a touch-induced release of ATP to the extracellular space. While sequential touch stimulation reve ...[more]

PMID: 19596005

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