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Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP.


ABSTRACT: Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein ? subunit (G?) (gpa1-1 and gpa1-2) and enhanced in G?-over-expression (OE) lines (wG? and cG?). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd3+ remarkably suppressed eATP-induced root bending. ATP-stimulated Ca2+ influx was impaired in G? null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in G? null mutants. In addition, some eATP-induced genes' expression was also impaired in G? null mutants. Based on these results, we propose that heterotrimeric G?-regulated Ca2+ influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots.

SUBMITTER: Zhu R 

PROVIDER: S-EPMC5585194 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Heterotrimeric G Protein-Regulated Ca<sup>2+</sup> Influx and PIN2 Asymmetric Distribution Are Involved in <i>Arabidopsis thaliana</i> Roots' Avoidance Response to Extracellular ATP.

Zhu Ruojia R   Dong Xiaoxia X   Hao Weiwei W   Gao Wei W   Zhang Wenzhu W   Xia Shuyan S   Liu Ting T   Shang Zhonglin Z  

Frontiers in plant science 20170901


Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of <i>Arabidopsis thaliana</i> seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (<i>gpa1-1  ...[more]

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