Ontology highlight
ABSTRACT:
SUBMITTER: Widmaier DM
PROVIDER: S-EPMC2758716 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Widmaier Daniel M DM Tullman-Ercek Danielle D Mirsky Ethan A EA Hill Rena R Govindarajan Sridhar S Minshull Jeremy J Voigt Christopher A CA
Molecular systems biology 20090915
The type III secretion system (T3SS) exports proteins from the cytoplasm, through both the inner and outer membranes, to the external environment. Here, a system is constructed to harness the T3SS encoded within Salmonella Pathogeneity Island 1 to export proteins of biotechnological interest. The system is composed of an operon containing the target protein fused to an N-terminal secretion tag and its cognate chaperone. Transcription is controlled by a genetic circuit that only turns on when the ...[more]