Unknown

Dataset Information

0

Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes.


ABSTRACT: To further understand the roles of protein glycosylation in eukaryotes, we globally identified glycan-containing proteins in yeast. A fluorescent lectin binding assay was developed and used to screen protein microarrays containing over 5000 proteins purified from yeast. A total of 534 yeast proteins were identified that bound either Concanavalin A (ConA) or Wheat-Germ Agglutinin (WGA); 406 of them were novel. Among the novel glycoproteins, 45 were validated by mobility shift upon treatment with EndoH and PNGase F, thereby extending the number of validated yeast glycoproteins to 350. In addition to many components of the secretory pathway, we identified other types of proteins, such as transcription factors and mitochondrial proteins. To further explore the role of glycosylation in mitochondrial function, the localization of four mitochondrial proteins was examined in the presence and absence of tunicamycin, an inhibitor of N-linked protein glycosylation. For two proteins, localization to the mitochondria is diminished upon tunicamycin treatment, indicating that protein glycosylation is important for protein function. Overall, our studies greatly extend our understanding of protein glycosylation in eukaryotes through the cataloguing of glycoproteins, and describe a novel role for protein glycosylation in mitochondrial protein function and localization.

SUBMITTER: Kung LA 

PROVIDER: S-EPMC2758718 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

altmetric image

Publications

Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes.

Kung Li A LA   Tao Sheng-Ce SC   Qian Jiang J   Smith Michael G MG   Snyder Michael M   Zhu Heng H  

Molecular systems biology 20090915


To further understand the roles of protein glycosylation in eukaryotes, we globally identified glycan-containing proteins in yeast. A fluorescent lectin binding assay was developed and used to screen protein microarrays containing over 5000 proteins purified from yeast. A total of 534 yeast proteins were identified that bound either Concanavalin A (ConA) or Wheat-Germ Agglutinin (WGA); 406 of them were novel. Among the novel glycoproteins, 45 were validated by mobility shift upon treatment with  ...[more]

Similar Datasets

| S-EPMC4824858 | biostudies-literature
| S-EPMC6314163 | biostudies-literature
| S-EPMC6785544 | biostudies-literature
| S-EPMC3169507 | biostudies-literature
| S-EPMC5638772 | biostudies-literature
| S-EPMC7876005 | biostudies-literature
| S-EPMC7002768 | biostudies-literature
| S-EPMC9092021 | biostudies-literature
| S-EPMC535686 | biostudies-literature
| S-EPMC4750496 | biostudies-literature