Ontology highlight
ABSTRACT:
SUBMITTER: Frisk ML
PROVIDER: S-EPMC2760437 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Frisk Megan L ML Tepp William H WH Johnson Eric A EA Beebe David J DJ
Analytical chemistry 20090401 7
A sensor for the lethal bacterial enzyme, botulinum neurotoxin type A (BoNT/A), was developed using self-assembled monolayers (SAMs). SAMs consisting of an immobilized synthetic peptide that mimicked the toxin's in vivo SNAP-25 protein substrate were formed on Au and interfaced with arrayed microfluidic channels. Efforts to optimize SAM composition and assay conditions for greatest reaction efficiency and sensitivity are described in detail. Channel design provided facile fluid manipulation, sam ...[more]