Ontology highlight
ABSTRACT:
SUBMITTER: Grizot S
PROVIDER: S-EPMC2760784 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Grizot Sylvestre S Smith Julianne J Daboussi Fayza F Prieto Jesús J Redondo Pilar P Merino Nekane N Villate Maider M Thomas Séverine S Lemaire Laetitia L Montoya Guillermo G Blanco Francisco J FJ Pâques Frédéric F Duchateau Philippe P
Nucleic acids research 20090707 16
Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to inactivate viruses, in a new approach to molecular medicine. However, such applications are highly demanding in terms of safety. Mutations in the human RAG1 gene cause severe combined immunodeficiency (SCID). Using the I-CreI dimeric LAGLIDADG meganuclease as a scaffold, we describe here the engineering o ...[more]