Ontology highlight
ABSTRACT:
SUBMITTER: Shu X
PROVIDER: S-EPMC2763207 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Shu Xiaokun X Royant Antoine A Lin Michael Z MZ Aguilera Todd A TA Lev-Ram Varda V Steinbach Paul A PA Tsien Roger Y RY
Science (New York, N.Y.) 20090501 5928
Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extincti ...[more]