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Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

ABSTRACT: The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650?nm and the onset of increased water absorption at 900?nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

SUBMITTER: Kamper M

PROVIDER: S-EPMC6232180 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

Kamper Maria M Ta Haisen H Jensen Nickels A NA Hell Stefan W SW Jakobs Stefan S

Nature communications 20181112 1

The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color re ...[more]

PMID: 30420676

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