Ontology highlight
ABSTRACT:
SUBMITTER: Salomonis N
PROVIDER: S-EPMC2764345 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Salomonis Nathan N Nelson Brandon B Vranizan Karen K Pico Alexander R AR Hanspers Kristina K Kuchinsky Allan A Ta Linda L Mercola Mark M Conklin Bruce R BR
PLoS computational biology 20091106 11
The role of alternative splicing in self-renewal, pluripotency and tissue lineage specification of human embryonic stem cells (hESCs) is largely unknown. To better define these regulatory cues, we modified the H9 hESC line to allow selection of pluripotent hESCs by neomycin resistance and cardiac progenitors by puromycin resistance. Exon-level microarray expression data from undifferentiated hESCs and cardiac and neural precursors were used to identify splice isoforms with cardiac-restricted or ...[more]