A shotgun proteomic method for the identification of membrane-embedded proteins and peptides.
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ABSTRACT:
SUBMITTER: Blackler AR
PROVIDER: S-EPMC2765231 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Publications
A shotgun proteomic method for the identification of membrane-embedded proteins and peptides.
Blackler Adele R AR Speers Anna E AE Ladinsky Mark S MS Wu Christine C CC
Journal of proteome research 20080607 7
Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome. ...[more]
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