An improved shotgun antisense method for mutagenesis and gene identification.
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ABSTRACT:
SUBMITTER: Tang Y
PROVIDER: S-EPMC7092706 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
An improved shotgun antisense method for mutagenesis and gene identification.
BioTechniques 20200124 3
Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in <i>Dictyostelium discoideum</i>. However, the method has two limitations. First, there were too few clones in the shotgun antisense cDNA library to have an antisense cDNA for every gene in the genome. Second, the unequal transcription level of genes resulted in many antisense cDNAs in the library for some genes but relatively few antisen ...[more]
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