Ontology highlight
ABSTRACT:
SUBMITTER: Waanders LF
PROVIDER: S-EPMC2765458 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Waanders Leonie F LF Chwalek Karolina K Monetti Mara M Kumar Chanchal C Lammert Eckhard E Mann Matthias M
Proceedings of the National Academy of Sciences of the United States of America 20091021 45
Technological developments make mass spectrometry (MS)-based proteomics a central pillar of biochemical research. MS has been very successful in cell culture systems, where sample amounts are not limiting. To extend its capabilities to extremely small, physiologically distinct cell types isolated from tissue, we developed a high sensitivity chromatographic system that measures nanogram protein mixtures for 8 h with very high resolution. This technology is based on splitting gradient effluents in ...[more]