Ontology highlight
ABSTRACT:
SUBMITTER: Bonnycastle LL
PROVIDER: S-EPMC6964677 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Bonnycastle Lori L LL Gildea Derek E DE Yan Tingfen T Narisu Narisu N Swift Amy J AJ Wolfsberg Tyra G TG Erdos Michael R MR Collins Francis S FS
Biology methods & protocols 20200116 1
Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and logistical challenges, especially for tissues that are only available at autopsy, which includes pancreatic islets, a tissue that is highly relevant to diabetes. To assess the possible effects of different sample preparation protocols on fresh islet samples, we ...[more]