De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data.
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ABSTRACT:
SUBMITTER: Diguistini S
PROVIDER: S-EPMC2768983 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data.
Diguistini Scott S Liao Nancy Y NY Platt Darren D Robertson Gordon G Seidel Michael M Chan Simon K SK Docking T Roderick TR Birol Inanc I Holt Robert A RA Hirst Martin M Mardis Elaine E Marra Marco A MA Hamelin Richard C RC Bohlmann Jörg J Breuil Colette C Jones Steven Jm SJ
Genome biology 20090911 9
Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest pathogen Grosmannia clavigera, an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read data and ...[more]
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