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A yeast two-hybrid smart-pool-array system for protein-interaction mapping.

ABSTRACT: We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.

SUBMITTER: Jin F 

PROVIDER: S-EPMC2771206 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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A yeast two-hybrid smart-pool-array system for protein-interaction mapping.

Jin Fulai F   Avramova Larisa L   Huang Jing J   Hazbun Tony T  

Nature methods 20070422 5

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude. ...[more]

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