DoMY-Seq: A yeast two-hybrid-based technique for precision mapping of protein-protein interaction motifs.

ABSTRACT: Interactions between proteins are fundamental for every biological process and especially important in cell signaling pathways. Biochemical techniques that evaluate these protein-protein interactions (PPIs), such as in vitro pull downs and coimmunoprecipitations, have become popular in most laboratories and are essential to identify and validate novel protein binding partners. Most PPIs occur through small domains or motifs, which are challenging and laborious to map by using standard biochemical approaches because they generally require the cloning of several truncation mutants. Moreover, these classical methodologies provide limited resolution of the interacting interface. Here, we describe the development of an alternative technique to overcome these limitations termed "Protein Domain m

SUBMITTER: Castel P 

PROVIDER: S-EPMC7949039 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature