Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol.
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ABSTRACT:
SUBMITTER: Trinh CT
PROVIDER: S-EPMC2772450 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
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Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol.
Trinh Cong T CT Srienc Friedrich F
Applied and environmental microbiology 20090904 21
Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that support cell function. Among these pathways are eight aerobic and eight anaerobic pathways that do not support cell growth but convert glycerol into ethanol with a theoretical yield ...[more]
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