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Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli.

ABSTRACT: We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

SUBMITTER: Andreessen B

PROVIDER: S-EPMC2805219 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli.

Andreessen Björn B Lange Alvin Brian AB Robenek Horst H Steinbüchel Alexander A

Applied and environmental microbiology 20091120 2

We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present ...[more]

PMID: 19933347

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