Unknown

Dataset Information

0

Adenoviral transfer of HIF-1alpha enhances vascular responses to critical limb ischemia in diabetic mice.


ABSTRACT: Diabetes is a major risk factor for ischemic disease. Treatment options for diabetic patients with peripheral arterial disease when revascularization is not possible are limited, resulting in a high incidence of limb amputation. We evaluated the therapeutic potential of AdCA5, an adenovirus encoding a constitutively active form of HIF-1alpha, in a diabetic model of critical limb ischemia. Diabetic db/db and nondiabetic db/+ mice were subjected to unilateral femoral artery ligation. Limb perfusion, tissue viability, and motor function were more severely impaired in db/db mice. Intramuscular injection of AdCA5 into the ischemic limb of db/db mice increased the recovery of limb perfusion and function, reduced tissue necrosis, rescued the diabetes-associated impairment of circulating angiogenic cells, enhanced endothelial nitric oxide synthase activation, and increased vessel density and luminal area in the ischemic limb.

SUBMITTER: Sarkar K 

PROVIDER: S-EPMC2774037 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Adenoviral transfer of HIF-1alpha enhances vascular responses to critical limb ischemia in diabetic mice.

Sarkar Kakali K   Fox-Talbot Karen K   Steenbergen Charles C   Bosch-Marcé Marta M   Semenza Gregg L GL  

Proceedings of the National Academy of Sciences of the United States of America 20091019 44


Diabetes is a major risk factor for ischemic disease. Treatment options for diabetic patients with peripheral arterial disease when revascularization is not possible are limited, resulting in a high incidence of limb amputation. We evaluated the therapeutic potential of AdCA5, an adenovirus encoding a constitutively active form of HIF-1alpha, in a diabetic model of critical limb ischemia. Diabetic db/db and nondiabetic db/+ mice were subjected to unilateral femoral artery ligation. Limb perfusio  ...[more]

Similar Datasets

| S-EPMC2614777 | biostudies-literature
| S-EPMC2787122 | biostudies-literature
| S-EPMC5997001 | biostudies-literature
| S-EPMC3281555 | biostudies-literature
2024-04-03 | PXD050360 | Pride
| S-EPMC2064828 | biostudies-other
| S-EPMC7047615 | biostudies-literature
| S-EPMC3761355 | biostudies-literature
| S-EPMC10790628 | biostudies-literature
| S-EPMC9163779 | biostudies-literature