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The thermo- and acido-stable ORF-99 from the archaeal virus AFV1.

ABSTRACT: Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues.

SUBMITTER: Goulet A

PROVIDER: S-EPMC2774441 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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The thermo- and acido-stable ORF-99 from the archaeal virus AFV1.

Goulet Adeline A Spinelli Silvia S Blangy Stéphanie S van Tilbeurgh Herman H Leulliot Nicolas N Basta Tamara T Prangishvili David D Cambillau Christian C Campanacci Valérie V

Protein science : a publication of the Protein Society 20090601 6

Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 ...[more]

PMID: 19472363

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