Ontology highlight
ABSTRACT:
SUBMITTER: Tandon N
PROVIDER: S-EPMC2775058 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Tandon Nina N Cannizzaro Christopher C Chao Pen-Hsiu Grace PH Maidhof Robert R Marsano Anna A Au Hoi Ting Heidi HT Radisic Milica M Vunjak-Novakovic Gordana G
Nature protocols 20090101 2
We describe a protocol for tissue engineering of synchronously contractile cardiac constructs by culturing cardiac cells with the application of pulsatile electrical fields designed to mimic those present in the native heart. Tissue culture is conducted in a customized chamber built to allow for cultivation of (i) engineered three-dimensional (3D) cardiac tissue constructs, (ii) cell monolayers on flat substrates or (iii) cells on patterned substrates. This also allows for analysis of the indivi ...[more]