Large-scale profiling of protein palmitoylation in mammalian cells.
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ABSTRACT:
SUBMITTER: Martin BR
PROVIDER: S-EPMC2775068 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
Large-scale profiling of protein palmitoylation in mammalian cells.
Martin Brent R BR Cravatt Benjamin F BF
Nature methods 20090111 2
S-palmitoylation is a pervasive post-translational modification required for the trafficking, compartmentalization and membrane tethering of many proteins. We demonstrate that the commercially available compound 17-octadecynoic acid (17-ODYA) can serve as a bioorthogonal, click chemistry probe for in situ labeling, identification and verification of palmitoylated proteins in human cells. We identified approximately 125 predicted palmitoylated proteins, including G proteins, receptors and a famil ...[more]
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