Ontology highlight
ABSTRACT:
SUBMITTER: Kim K
PROVIDER: S-EPMC2777445 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Nucleic acids research 20090903 20
Magnetic tweezers (MT) are single-molecule manipulation instruments that utilize a magnetic field to apply force to a biomolecule-tethered magnetic bead while using optical bead tracking to measure the biomolecule's extension. While relatively simple to set up, prior MT implementations have lacked the resolution necessary to observe sub-nanometer biomolecular configuration changes. Here, we demonstrate a reflection-interference technique for bead tracking, and show that it has much better resolu ...[more]