Unknown

Dataset Information

0

Genome-wide analysis of chromatin regulation by cocaine reveals a role for sirtuins.


ABSTRACT: Changes in gene expression contribute to the long-lasting regulation of the brain's reward circuitry seen in drug addiction; however, the specific genes regulated and the transcriptional mechanisms underlying such regulation remain poorly understood. Here, we used chromatin immunoprecipitation coupled with promoter microarray analysis to characterize genome-wide chromatin changes in the mouse nucleus accumbens, a crucial brain reward region, after repeated cocaine administration. Our findings reveal several interesting principles of gene regulation by cocaine and of the role of DeltaFosB and CREB, two prominent cocaine-induced transcription factors, in this brain region. The findings also provide comprehensive insight into the molecular pathways regulated by cocaine-including a new role for sirtuins (Sirt1 and Sirt2)-which are induced in the nucleus accumbens by cocaine and, in turn, dramatically enhance the behavioral effects of the drug.

SUBMITTER: Renthal W 

PROVIDER: S-EPMC2779727 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

altmetric image

Publications


Changes in gene expression contribute to the long-lasting regulation of the brain's reward circuitry seen in drug addiction; however, the specific genes regulated and the transcriptional mechanisms underlying such regulation remain poorly understood. Here, we used chromatin immunoprecipitation coupled with promoter microarray analysis to characterize genome-wide chromatin changes in the mouse nucleus accumbens, a crucial brain reward region, after repeated cocaine administration. Our findings re  ...[more]

Similar Datasets

| S-EPMC3113561 | biostudies-literature
| S-EPMC3335379 | biostudies-literature
2010-05-19 | E-GEOD-15968 | biostudies-arrayexpress
2009-05-20 | GSE15968 | GEO
| S-EPMC3753870 | biostudies-literature
| S-EPMC2709841 | biostudies-literature
| S-EPMC4615903 | biostudies-literature
2010-05-18 | E-GEOD-16183 | biostudies-arrayexpress
2010-05-18 | E-GEOD-16184 | biostudies-arrayexpress
2009-05-20 | GSE16184 | GEO