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Microfluidic device for single-molecule experiments with enhanced photostability.

ABSTRACT: A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

SUBMITTER: Lemke EA

PROVIDER: S-EPMC2779842 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

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Microfluidic device for single-molecule experiments with enhanced photostability.

Lemke Edward A EA Gambin Yann Y Vandelinder Virginia V Brustad Eric M EM Liu Hsiao-Wei HW Schultz Peter G PG Groisman Alex A Deniz Ashok A AA

Journal of the American Chemical Society 20090901 38

A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents. ...[more]

PMID: 19772358

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