Ontology highlight
ABSTRACT:
SUBMITTER: Basu S
PROVIDER: S-EPMC6023872 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Basu Srinjan S Needham Lisa-Maria LM Lando David D Taylor Edward J R EJR Wohlfahrt Kai J KJ Shah Devina D Boucher Wayne W Tan Yi Lei YL Bates Lawrence E LE Tkachenko Olga O Cramard Julie J Lagerholm B Christoffer BC Eggeling Christian C Hendrich Brian B Klenerman Dave D Lee Steven F SF Laue Ernest D ED
Nature communications 20180628 1
A major challenge in single-molecule imaging is tracking the dynamics of proteins or complexes for long periods of time in the dense environments found in living cells. Here, we introduce the concept of using FRET to enhance the photophysical properties of photo-modulatable (PM) fluorophores commonly used in such studies. By developing novel single-molecule FRET pairs, consisting of a PM donor fluorophore (either mEos3.2 or PA-JF<sub>549</sub>) next to a photostable acceptor dye JF<sub>646</sub> ...[more]