MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays.
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ABSTRACT:
SUBMITTER: Schuffler P
PROVIDER: S-EPMC2784320 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays.
Schüffler Peter P Mikeska Thomas T Waha Andreas A Lengauer Thomas T Bock Christoph C
Genome biology 20091005 10
DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://methmarker.mpi-inf.mpg.de/ software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore, MethMarker implements a bioinformatic workflow for transfo ...[more]
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