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Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels.

ABSTRACT: Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived strain A/J, and the Mus musculus castaneus derived strain CAST/Ei. We describe approaches for the accurate identification of nucleotide and structural variation in the genomes of vertebrate experimental organisms, and show how these techniques can be applied to help prioritize candidate genes within quantitative trait loci.

SUBMITTER: Sudbery I

PROVIDER: S-EPMC2784327 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels.

Sudbery Ian I Stalker Jim J Simpson Jared T JT Keane Thomas T Rust Alistair G AG Hurles Matthew E ME Walter Klaudia K Lynch Dee D Teboul Lydia L Brown Steve D SD Li Heng H Ning Zemin Z Nadeau Joseph H JH Croniger Colleen M CM Durbin Richard R Adams David J DJ

Genome biology 20091013 10

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived strain A/J, and the Mus musculus castaneus derived strain CAST/Ei. We describe approaches for the accurate identification of nucleotide and structural variation in the genomes of vertebrate experimental organisms, and show how these techniques can be applied to help prioritize candidate genes within quantitative trai ...[more]

PMID: 19825173

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