Ontology highlight
ABSTRACT:
SUBMITTER: Zheng GX
PROVIDER: S-EPMC4786454 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Zheng Grace X Y GX Lau Billy T BT Schnall-Levin Michael M Jarosz Mirna M Bell John M JM Hindson Christopher M CM Kyriazopoulou-Panagiotopoulou Sofia S Masquelier Donald A DA Merrill Landon L Terry Jessica M JM Mudivarti Patrice A PA Wyatt Paul W PW Bharadwaj Rajiv R Makarewicz Anthony J AJ Li Yuan Y Belgrader Phillip P Price Andrew D AD Lowe Adam J AJ Marks Patrick P Vurens Gerard M GM Hardenbol Paul P Montesclaros Luz L Luo Melissa M Greenfield Lawrence L Wong Alexander A Birch David E DE Short Steven W SW Bjornson Keith P KP Patel Pranav P Hopmans Erik S ES Wood Christina C Kaur Sukhvinder S Lockwood Glenn K GK Stafford David D Delaney Joshua P JP Wu Indira I Ordonez Heather S HS Grimes Susan M SM Greer Stephanie S Lee Josephine Y JY Belhocine Kamila K Giorda Kristina M KM Heaton William H WH McDermott Geoffrey P GP Bent Zachary W ZW Meschi Francesca F Kondov Nikola O NO Wilson Ryan R Bernate Jorge A JA Gauby Shawn S Kindwall Alex A Bermejo Clara C Fehr Adrian N AN Chan Adrian A Saxonov Serge S Ness Kevin D KD Hindson Benjamin J BJ Ji Hanlee P HP
Nature biotechnology 20160201 3
Haplotyping of human chromosomes is a prerequisite for cataloguing the full repertoire of genetic variation. We present a microfluidics-based, linked-read sequencing technology that can phase and haplotype germline and cancer genomes using nanograms of input DNA. This high-throughput platform prepares barcoded libraries for short-read sequencing and computationally reconstructs long-range haplotype and structural variant information. We generate haplotype blocks in a nuclear trio that are concor ...[more]