Unknown

Dataset Information

0

C-Myb is required for pro-B cell differentiation.


ABSTRACT: The c-Myb transcription factor is required for normal adult hematopoiesis. However, the embryonic lethality of Myb-null mutations has been an impediment to identifying roles for c-Myb during lymphocyte development. We have used tissue-specific inactivation of the Myb locus in early progenitor cells to demonstrate that c-Myb is absolutely required for the differentiation of CD19(+) B-lineage cells and B cell differentiation is profoundly blocked beyond the pre-pro-B cell stage in Myb(f/f) Mb1-cre mice. We demonstrate that c-Myb is required for the intrinsic survival of CD19(+) pro-B cells as well as the proper expression of the alpha-chain of the IL-7 receptor (CD127) and Ebf1. However, survival of c-Myb-deficient CD19(+) pro-B cells cannot be rescued by transduction with CD127-producing retrovirus, suggesting that c-Myb controls a survival pathway independent of CD127. Furthermore, c-Myb-deficient progenitor cells inefficiently generate CD19(+) B-lineage cells during stromal cell culture but this process can be partially rescued with exogenous Ebf1. Thus, c-Myb does not appear to be required for commitment to B cell differentiation but is crucial for B cell differentiation to the CD19(+) pro-B cell stage as well as survival of CD19(+) pro-B cells. Surprisingly, forced c-Myb expression in lymphoid-primed multipotent progenitors favors differentiation toward the myeloid lineage, suggesting that proper c-Myb expression is crucial for B-lineage development.

SUBMITTER: Fahl SP 

PROVIDER: S-EPMC2785544 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

c-Myb is required for pro-B cell differentiation.

Fahl Shawn P SP   Crittenden Rowena B RB   Allman David D   Bender Timothy P TP  

Journal of immunology (Baltimore, Md. : 1950) 20091101 9


The c-Myb transcription factor is required for normal adult hematopoiesis. However, the embryonic lethality of Myb-null mutations has been an impediment to identifying roles for c-Myb during lymphocyte development. We have used tissue-specific inactivation of the Myb locus in early progenitor cells to demonstrate that c-Myb is absolutely required for the differentiation of CD19(+) B-lineage cells and B cell differentiation is profoundly blocked beyond the pre-pro-B cell stage in Myb(f/f) Mb1-cre  ...[more]

Similar Datasets

| S-EPMC4245327 | biostudies-literature
| S-EPMC4009620 | biostudies-literature
| S-EPMC5389521 | biostudies-literature
| S-EPMC1820669 | biostudies-literature
| S-EPMC3113568 | biostudies-literature
| S-EPMC5611819 | biostudies-literature
2013-06-18 | E-GEOD-44405 | biostudies-arrayexpress
| S-EPMC4359250 | biostudies-literature
2013-06-18 | GSE44405 | GEO
| S-EPMC1895449 | biostudies-literature