BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.
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ABSTRACT:
SUBMITTER: Yutin N
PROVIDER: S-EPMC2786397 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.
Yutin Natalya N Suzuki Marcelino T MT Rosenberg Mira M Rotem Denisse D Madigan Michael T MT Süling Jörg J Imhoff Johannes F JF Béjà Oded O
Applied and environmental microbiology 20091002 23
To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA. ...[more]
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