Ontology highlight
ABSTRACT:
SUBMITTER: Lee JE
PROVIDER: S-EPMC2786887 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Lee Ji-Eun JE Oum Boo Sup BS Choi Hee Young HY Lee Seung Uk SU Lee Jong Soo JS
Molecular vision 20091128
<h4>Purpose</h4>To identify the differentially expressed genes (DEGs) in the human keratocytes in keratoconus.<h4>Methods</h4>Total RNA extracted from cultured corneal stromal fibroblasts from normal and keratoconic corneas were used for the synthesis of cDNA. DEGs were screened by an annealing control primer(TM)-based PCR method using GeneFishing() DEG kits. The differentially expressed bands were sequenced and analyzed. The genes identified were further evaluated by reverse transcriptase PCR a ...[more]