Characterization of expression, activity and role in antibacterial immunity of Anopheles gambiae lysozyme c-1.
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ABSTRACT:
SUBMITTER: Kajla MK
PROVIDER: S-EPMC2795013 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
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Characterization of expression, activity and role in antibacterial immunity of Anopheles gambiae lysozyme c-1.
Kajla Mayur K MK Andreeva Olga O Gilbreath Thomas M TM Paskewitz Susan M SM
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 20100201 2
There are eight lysozyme genes in the Anopheles gambiae genome. Transcripts of one of these genes, LYSC-1, increased in Anopheles gambiae cell line 4a3B by 24 h after exposure to heat-killed Micrococcus luteus. Lysozyme activity was also identified in conditioned media from the cell line from which the protein was purified to homogeneity using ion exchange and gel filtration. Mass spectrometric analysis of the purified protein showed 100% identity to lysozyme c-1. Purified lysozyme c-1 was teste ...[more]
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