Competition between Co(NH(3)(6)3+ and inner sphere Mg2+ ions in the HDV ribozyme.
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ABSTRACT:
SUBMITTER: Gong B
PROVIDER: S-EPMC2795014 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
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Competition between Co(NH(3)(6)3+ and inner sphere Mg2+ ions in the HDV ribozyme.
Gong Bo B Chen Jui-Hui JH Bevilacqua Philip C PC Golden Barbara L BL Carey Paul R PR
Biochemistry 20091201 50
Divalent cations play critical structural and functional roles in many RNAs. While the hepatitis delta virus (HDV) ribozyme can undergo self-cleavage in the presence of molar concentrations of monovalent cations, divalent cations such as Mg(2+) are required for efficient catalysis under physiological conditions. Moreover, the cleavage reaction can be inhibited with Co(NH(3))(6)(3+), an analogue of Mg(H(2)O)(6)(2+). Here, the binding of Mg(2+) and Co(NH(3))(6)(3+) to the HDV ribozyme is studied b ...[more]
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