Translesion synthesis of abasic sites by yeast DNA polymerase epsilon.
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ABSTRACT:
SUBMITTER: Sabouri N
PROVIDER: S-EPMC2797225 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Publications
Translesion synthesis of abasic sites by yeast DNA polymerase epsilon.
Sabouri Nasim N Johansson Erik E
The Journal of biological chemistry 20090923 46
Studies of replicative DNA polymerases have led to the generalization that abasic sites are strong blocks to DNA replication. Here we show that yeast replicative DNA polymerase epsilon bypasses a model abasic site with comparable efficiency to Pol eta and Dpo4, two translesion polymerases. DNA polymerase epsilon also exhibited high bypass efficiency with a natural abasic site on the template. Translesion synthesis primarily resulted in deletions. In cases where only a single nucleotide was inser ...[more]
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