Unknown

Dataset Information

0

Roles of Tom70 in import of presequence-containing mitochondrial proteins.


ABSTRACT: Mitochondrial protein traffic requires precise recognition of the mitochondrial targeting signals by the import receptors on the mitochondrial surface including a general import receptor Tom20 and a receptor for presequence-less proteins, Tom70. Here we took a proteome-wide approach of mitochondrial protein import in vitro to find a set of presequence-containing precursor proteins for recognition by Tom70. The presequences of the Tom70-dependent precursor proteins were recognized by Tom20, whereas their mature parts exhibited Tom70-dependent import when attached to the presequence of Tom70-independent precursor proteins. The mature parts of the Tom70-dependent precursor proteins have the propensity to aggregate, and the presence of the receptor domain of Tom70 prevents their aggregate formation. Therefore Tom70 plays the role of a docking site for not only cytosolic chaperones but also aggregate-prone substrates to maintain their solubility for efficient transfer to downstream components of the mitochondrial import machineries.

SUBMITTER: Yamamoto H 

PROVIDER: S-EPMC2797234 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Roles of Tom70 in import of presequence-containing mitochondrial proteins.

Yamamoto Hayashi H   Fukui Kenji K   Takahashi Hisashi H   Kitamura Shingo S   Shiota Takuya T   Terao Kayoko K   Uchida Mayumi M   Esaki Masatoshi M   Nishikawa Shuh-ichi S   Yoshihisa Tohru T   Yamano Koji K   Endo Toshiya T  

The Journal of biological chemistry 20090918 46


Mitochondrial protein traffic requires precise recognition of the mitochondrial targeting signals by the import receptors on the mitochondrial surface including a general import receptor Tom20 and a receptor for presequence-less proteins, Tom70. Here we took a proteome-wide approach of mitochondrial protein import in vitro to find a set of presequence-containing precursor proteins for recognition by Tom70. The presequences of the Tom70-dependent precursor proteins were recognized by Tom20, where  ...[more]

Similar Datasets

| S-EPMC5026490 | biostudies-literature
| S-EPMC5881500 | biostudies-literature
| S-EPMC1951752 | biostudies-literature
| S-EPMC8605242 | biostudies-literature
| S-EPMC2246612 | biostudies-literature
| S-EPMC7962155 | biostudies-literature
| S-EPMC3173181 | biostudies-literature
| S-EPMC5009240 | biostudies-literature
| S-EPMC4816344 | biostudies-literature
| S-EPMC4878746 | biostudies-literature