Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.
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ABSTRACT:
SUBMITTER: Anbarasan S
PROVIDER: S-EPMC2798631 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
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Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.
Anbarasan Sasikala S Jänis Janne J Paloheimo Marja M Laitaoja Mikko M Vuolanto Minna M Karimäki Johanna J Vainiotalo Pirjo P Leisola Matti M Turunen Ossi O
Applied and environmental microbiology 20091023 1
The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH. ...[more]
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