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Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.


ABSTRACT: The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

SUBMITTER: Anbarasan S 

PROVIDER: S-EPMC2798631 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.

Anbarasan Sasikala S   Jänis Janne J   Paloheimo Marja M   Laitaoja Mikko M   Vuolanto Minna M   Karimäki Johanna J   Vainiotalo Pirjo P   Leisola Matti M   Turunen Ossi O  

Applied and environmental microbiology 20091023 1


The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH. ...[more]

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