Ontology highlight
ABSTRACT:
SUBMITTER: Heeres JT
PROVIDER: S-EPMC2799191 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Heeres James T JT Kim Seok-Ho SH Leslie Benjamin J BJ Lidstone Erich A EA Cunningham Brian T BT Hergenrother Paul J PJ
Journal of the American Chemical Society 20091201 51
Inhibitors and activators of protein-protein interactions are valuable as biological probes and medicinal agents but are often difficult to identify. Herein we describe a high-throughput assay, based upon photonic crystal (PC) biosensors, for the identification of modulators of protein-protein interactions. Through the use of a d-biotin-tris-NTA (BTN) hybrid compound, any His6-tagged protein can be immobilized on the surface of a PC biosensor. Binding of the bound protein to its cognate partner ...[more]