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Structure of D-lactate dehydrogenase from Aquifex aeolicus complexed with NAD(+) and lactic acid (or pyruvate).

ABSTRACT: The crystal structure of D-lactate dehydrogenase from Aquifex aeolicus (aq_727) was determined to 2.12 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 90.94, b = 94.43, c = 188.85 A. The structure was solved by molecular replacement using the coenzyme-binding domain of Lactobacillus helveticus D-lactate dehydrogenase and contained two homodimers in the asymmetric unit. Each subunit of the homodimer was found to be in a ;closed' conformation with the NADH cofactor bound to the coenzyme-binding domain and with a lactate (or pyruvate) molecule bound at the interdomain active-site cleft.

SUBMITTER: Antonyuk SV

PROVIDER: S-EPMC2802865 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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Structure of D-lactate dehydrogenase from Aquifex aeolicus complexed with NAD(+) and lactic acid (or pyruvate).

Antonyuk Svetlana V SV Strange Richard W RW Ellis Mark J MJ Bessho Yoshitaka Y Kuramitsu Seiki S Inoue Yumiko Y Yokoyama Shigeyuki S Hasnain S Samar SS

Acta crystallographica. Section F, Structural biology and crystallization communications 20091127 Pt 12

The crystal structure of D-lactate dehydrogenase from Aquifex aeolicus (aq_727) was determined to 2.12 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 90.94, b = 94.43, c = 188.85 A. The structure was solved by molecular replacement using the coenzyme-binding domain of Lactobacillus helveticus D-lactate dehydrogenase and contained two homodimers in the asymmetric unit. Each subunit of the homodimer was found to be in a ;closed' conformation with the NADH cofactor bound t ...[more]

PMID: 20054113

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Crystal structure of human aldehyde dehydrogenase 1A3 complexed with NAD+ and retinoic acid.

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Structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus.

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Expression, purification and X-ray analysis of 1,3-propanediol dehydrogenase (Aq_1145) from Aquifex aeolicus VF5.

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